Statistics of the Database


Table 1. Statistics of the RNA-Seq tags
 
P. falciparum and Human

patient# of non-splice tags   # of splice tags


human P. falciparumhuman P. falciparum

A14,537,848 39,8282,029,936 41
B 9,229,842 916,0431,216,4491,128
C10,354,8611,421,3071,394,6021,491


T. gondii and Human

Time of induction# of non-splice tags   # of splice tags


human T. gondiihuman T. gondii

0h 13,654,489129,9052,301,903 3,556
6h 14,513,825192,2592,448,076 5,484
24h 14,282,929267,0712,304,199 7,762
72h 13,450,092628,6642,080,56021,775
144h 14,003,765770,3961,980,67325,127
24h (not induced) 5,215,606460,607 565,950 3,540

Table 2. Statistics of the 5'EST and Shotgun Sequences
(A) Full-Parasites and (B) Full-Arthropods
 
Table 2A

SpeciesStage# ESTs# shotgun
seqeunced/locus
# total tags# sequence assembled
gap closed*1/locus
# sequence assembled
without gaps/locus
# average coverage*2

P. falciparumerythrocytic11,762 4,229/ 2,847  25,866,7781,482/1,239 348/ 330x39
P. vivax erythrocytic13,501 3,504/ 2,659  45,322,4781,871/1,4391,256/1,063x247
P. yoelii erythrocytic13,955 2,892/ 2,181  31,444,3981,130/ 983 795/ 713x417
P. berghei erythrocytic 1,275 678/ 573  30,791,291 211/ 178 138/ 126x1541
T. gondii tachyzoite 9,862 2,213/ 1,390  16,742,5651,244/ 871 865/ 662x239
C. parvum sporozite 11,873 1,302/ 851  18,319,304 713/ 512 557/ 426x249

Total 62,22814,818/10,501 168,486,8146,651/5,2223,959/3,320ND

 
Table 2B

SpeciesStage# ESTs# shotgun
seqeunced/locus
# total tags# sequence assembled
gap closed*1/locus
# sequence assembled
without gaps/locus
# average coverage*2

A. stephensilarva 12,590 4,053/ 2,225  23,023,172 ND1,054/2,802x115
G. morsitanslarva/pupa 14,713 6,346/ 2,596  29,343,254 ND4,973/2,062x59
D. farinae adult 23,040 ND  ND ND NDND

Total 50,34310,399/ 4,821  52,366,426 ND7,775/3,116ND

*1 Number of assembles were counted for which an open reading frame of the amino acids of >100 amino acids was detected. Note that for Full-Anopheles, gap closure was not performed due to the lack of the reference genome information.
*2 Coverage was calculated against the "sequence assembled gap closed" population.
Table 3. Statistics of the TSS tags
(A) Full-Parasites and (B) Full-Arthropods
 
Table 3A

Species Stage # TSS tags #mapped TSS positions
/# total mapped TSS tags
# corresponded genes*1

T. gondii bradizoite 6,801,945 104,926/2,739,596 5,647

 
Table 3B

Species Stage # TSS tags #mapped TSS positions
/# total mapped TSS tags
# corresponded genes*1

A. stephensi larva 8,354,743 21,897/ 97,395  542
G. morsitanslarva 5,734,822 129,706/1,519,515  1,961
pupa 8,330,172 149,861/2,434,906 

*1 Numbers of corresponded genes were counted for which TSS tags were mapped to the genic region (-1kb to the 3'-end) of the annotated genes in T. gondii and to the assembled cDNA sequences in A. stephensi and G. morsitans.